Abstract:
Magnetite is a common product of bacterial iron reduction and may serve as a potential physical indicator of biological activity in geological settings. Here we report the formation of single-domain magnetite under laboratory conditions by a thermophilic fermentative bacterial strain TOR-39 that was isolated from the deep subsurface. Time-course analyses were performed at 65 degrees C to study the effect of bacterial activity on solution chemistry and magnetite formation during the growth of TOR-39. Run products were examined by transmission electron microscopy. Magnetite particles formed exclusively outside of bacterial cells and exhibited octahedral shapes having relatively equal length and width (<15% difference). Tiny magnetite particles (<12 nm) nucleated between 10 and 11 h of incubation and increased to average lengths of 55.4 + or - 26.8 nm after 24 h of incubation. Between 24 h and 22 d of incubation, magnetite particles maintained average lengths of 56.2 + or - 24.8 nm. Based on size constraints, greater than 85% of the particles observed fell within the magnetic single domain. Little to no magnetite was detected in abiotic controls at 65 or 95 degrees C, or in TOR-39 cultures whose activity was suppressed. Unlike mesophilic iron-reducing bacteria (e.g., GS-15), TOR-39 produced crystals having shapes and sizes similar to some particles produced intracellularly by magnetotactic bacteria. Thus the single-domain magnetite produced by thermophiles such as TOR-39 may represent a heretofore unrecognized biological contribution to natural remanent magnetization in sedimentary basins and other geothermal environments.