MECHANISMS OF URANIUM MINERALIZATION BY THE YEAST SACCHAROMYCES CEREVISIAE
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dc.contributor.author | Ohnuki T. | |
dc.contributor.author | Ozaki T. | |
dc.contributor.author | Yoshida T. | |
dc.contributor.author | Sakamoto F. | |
dc.contributor.author | Kozai N. | |
dc.contributor.author | Wakai E. | |
dc.contributor.author | Francis A.J. | |
dc.contributor.author | Iefuji H. | |
dc.date.accessioned | 2023-12-23T04:12:18Z | |
dc.date.available | 2023-12-23T04:12:18Z | |
dc.date.issued | 2005 | |
dc.identifier | https://www.elibrary.ru/item.asp?id=31281644 | |
dc.identifier.citation | Geochimica et Cosmochimica Acta, 2005, 69, 22, 5307-5316 | |
dc.identifier.issn | 0016-7037 | |
dc.identifier.uri | https://repository.geologyscience.ru/handle/123456789/42381 | |
dc.description.abstract | We determined the association of uranium in yeast cells S. cerevisiae grown in medium containing high (1 g · L-1) or low (0.2 g · L-1) concentrations of phosphate after exposure for 96 h to a 4 × 10-4 mol · L-1 U(VI) solution at pH 3.2 or 4.7. The analysis was made using a field emission scanning electron microscope equipped with energy dispersive spectroscopy (FESEM-EDS), transmission electron microscopy (TEM), and visible diffuse reflectance spectrometry. Cells grown in the high-phosphate medium rapidly accumulated U(VI) from solution at pH 3.2 over the first 24 h, followed by a slow uptake until 96 h, whereas in cells grown in low-phosphate medium, U(VI) accumulation reached a steady state within 24 h. FESEM-EDS analyses revealed the formation of a U(VI)-bearing precipitate on the yeast cells grown in high-phosphate medium after only 48 h exposure; no precipitate was detected on cells grown in low-phosphate medium up to 96 h. These results suggest that sorption onto the cell surfaces was the dominant process initially. Analysis of the U(VI)-bearing precipitates by all three methods demonstrated the presence of H-autunite, HUO2PO4 · 4H2O. Thermodynamic calculations suggest that the chemical compositions of the solutions containing yeast grown in high-phosphate medium were undersaturated with respect to H-autunite, but were supersaturated with ten times more U(VI) and P than were actually observed. Apparently, the sorbed U(VI) on the cell surfaces reacts with P released from the yeast to form H-autunite by local saturation. The U(VI) uptake by yeast cells grown in high phosphate medium at pH 4.7, along with the thermodynamic calculation, indicated that more H-autunite is precipitated in neutral pH solution than in acid solution. Thus, U(VI)-phosphate mineralization on the cells of microorganisms should be taken into account for predicting U(VI) mobility in the environment. Copyright © 2005 Elsevier Ltd. | |
dc.subject | uranium mineralization | |
dc.title | MECHANISMS OF URANIUM MINERALIZATION BY THE YEAST SACCHAROMYCES CEREVISIAE | |
dc.type | Статья | |
dc.identifier.doi | 10.1016/j.gca.2005.06.023 |
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