GAS CHROMATOGRAPHY-PYROLYSIS-ISOTOPE RATIO MASS SPECTROMETRY: A NEW METHOD FOR INVESTIGATING INTRAMOLECULAR ISOTOPIC VARIATION IN LOW MOLECULAR WEIGHT ORGANIC ACIDS

Abstract

A new GC-Pyrolysis-IRMS method was developed for the δ13C determination of carboxyl carbon in low-molecular weight organic acids. By utilizing a palladium-wire reactor at 600<space>°C with a helium/hydrogen reactant gas, the carboxyl carbon of low-molecular weight organic acids is pyrolytically cleaved and introduced into an IRMS for stable carbon-isotope analysis. The precision of the GC-Py-IRMS method is similar to that of more conventional, combustion-based continuous-flow IRMS techniques and interpretation of isotope-dilution experiments with acetic and octanoic acid shows that the technique is sufficiently accurate for the determination of δ13C values at natural abundance levels. As a demonstration of this new capability, the carboxyl carbon of low-molecular weight (LMW, C2-C6) organic acids generated via hydrous pyrolysis of an oil-prone source rock (the Ghareb Shale) shows δ13C values consistent with the hypothesis that organic acids readily undergo exchange of their carboxyl carbon with aqueous inorganic carbon.